Figure 2 | Scientific Reports

Figure 2

From: Characterization of the interaction between Toxoplasma gondii rhoptry neck protein 4 and host cellular β-tubulin

Figure 2

The C-terminal half of TgRON4 binds to host β-tubulin.

(a) Schematic representation of TgRON4 and its deletion mutants. (b), (c) Coimmunoprecipitation of β-tubulin with deletion mutants of 3xFLAG-tagged TgRON4. 293 T (b) and Vero (c) cells were transiently transfected with the indicated TgRON4 expression plasmids. Membrane fractions were immunoprecipitated with an anti-β-tubulin antibody. The membrane fractions (3.3% input) and immunoprecipitates (IP) were analyzed by Western blotting with anti-FLAG and anti-β-tubulin antibodies.

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