Figure 4 | Scientific Reports

Figure 4

From: Characterization of the interaction between Toxoplasma gondii rhoptry neck protein 4 and host cellular β-tubulin

Figure 4

Identification of the TUBB2C-binding region in TgRON4.

(a) Schematic representation of TgRON4B and its deletion mutants. (b), (c) The indicated 3xFLAG-tagged RON4 proteins were coexpressed with HA-tagged TUBB2C F3R in Vero and CHO cells. The cell lysates were immunoprecipitated with an anti-FLAG antibody. The cell lysates and immunoprecipitates (IP) were analyzed by Western blotting with anti-HA and anti-FLAG antibodies.

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