Figure 3 | Scientific Reports

Figure 3

From: Defining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies

Figure 3

Generation of Hu-NSG mice EBV model and staining of infected mouse spleen and clinical NPC biopsies with TCR-like mAbs.

(a) Experimental workflow of the Hu-NSG mice EBV model -isolation of HSC; injection into NSG mice; flow cytometric analysis of reconstitution; EBV infection of Hu-NSG mice; and development of splenic lesions. The reconsitution of the Hu-NSG mice with human immune cells was approximately 65% ± 3.9%. (b) Spleens of EBV infected HLA-A0201 Hu-NSG mice showed positive red staining for TCR-like mAbs specific to LMP1, LMP2A and EBNA1 binding. Scale bars, 50 μm. (c) Immunological staining of NPC biopsy. Control panel was stained with secondary antibody. BB7.2 antibody stained for HLA-A02. The other two panels were stained with anti-HLA-A0201/LMP2A and anti-HLA-A0201/EBNA1 mAb. Positive staining was observed to be reddish brown. Scale bars, 25 μm.

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