Figure 1 | Scientific Reports

Figure 1

From: Glutathionylation potentiates benign superoxide dismutase 1 variants to the toxic forms associated with amyotrophic lateral sclerosis

Figure 1

Mass spectrometry analysis of SOD1 variants.

(a) Percentage of recombinantly expressed SOD1 that was glutathionylated (yellow), as determined by the area under the peaks of MS spectra, compared to disease progression (grey), expressed in truncated life years as loge[(75 − (25 + d)], where d equals the reported average disease duration, assumed life expectancy is 75 years and age at onset of disease is set to 50 years. (b) Mass spectrum of native SOD1WT (main) and SOD1A4V (faint) with species charge states labeled (D = dimer, M = monomer). Additional maxima within the peaks are the result of glutathionylation of one or both subunits to give a mixed (*) or gsSOD1(**) dimer. Inset shows the ratio of dimer to monomer for the two proteins, derived from charge state peak areas in the spectrum. (c) Expansion of the M7+ region showing peaks corresponding to uSOD1 (red) and gsSOD1 (yellow). (d) The percentage of gsSOD1 monomer (yellow) compared to disease progression (grey) for all SOD1 variants. The gsSOD1 measurements were performed in triplicate and error bars represent standard error of the mean.

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