Figure 1 | Scientific Reports

Figure 1

From: Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris

Figure 1

Och1p in N-glycan biosynthesis.

In the Golgi, the α-1,6-mannosyltransferase activity (α-1,6-MnT) of Och1p extends the N-linked Man8GlcNAc2 core glycan, which is then heterogeneously hyperglycosylated by several additional (phospho-) mannosyltransferases (MnTs). Galanthus nivalis lectin (GNL) and Lens culinaris lectin (LCA) bind to the different glycan structures either with high (thick arrow) or low (thin arrow) specificity.

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