Figure 6 | Scientific Reports

Figure 6

From: Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris

Figure 6

Quantitative determination of lectin binding on PpMutS and PpFWK3 cells.

Cell suspensions adjusted to the same concentration level were incubated with FITC labeled lectins. After thorough washing, cells were lysed and the fluorescence intensity of the lysis buffer recorded. Binding data was normalized to the average cellular content of FM® 4–64, which was shown to be similar in both strains via FACS analysis. Values represent mean ± SD of three independent experiments.

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