Table 2 Strain specific parameters of the different P. pastoris strains. Parameters are defined in the footnote^x

	PpMutS	PpMutS^HRP	PpFWK3	PpFWK3^HRP
max. μ_Gly (h⁻¹)	0.30	0.31	0.20	0.20
q_Gly (mmol·g⁻¹·h⁻¹)	2.90	3.10	1.90	1.90
Y_X/Gly (Cmol·Cmol⁻¹)	0.63	0.41	0.61	0.54
Y_CO2/Gly (Cmol·Cmol⁻¹)	0.33	0.64	0.37	0.44
Δtime _adapt (h)	15.7	19.9	6.60	8.90
q_MeOH (mmol·g⁻¹·h⁻¹)	0.62	0.70	0.52	0.43
max. q_MeOH-(mmol·g⁻¹·h⁻¹)	0.67	0.78	0.69	0.53
Y_X/MeOH (Cmol·Cmol⁻¹)	0.39	0.07	0.05	0.04
Y_CO2/MeOH (Cmol·Cmol⁻¹)	0.57	1.02	constantly decreasing	constantly decreasing
C-balance	0.97	1.04	constantly decreasing	constantly decreasing
q_p (U·g⁻¹·h⁻¹)	-	0.77	-	0.50
vol. productivity (U·L⁻¹·h⁻¹)	-	2.60	-	1.80
efficiency factor (η) (U·mmol⁻¹ ²⁶)	-	1.10	-	1.20

^Xmax. μ_Gly, maximum specific growth rate on glycerol; q_Gly, specific uptake rate of glycerol during the batch; Y_X/Gly, biomass yield on glycerol; Y_CO2/Gly, CO₂ yield on glycerol; Δtime_adapt, time from first addition of methanol to a maximum in offgas activity; q_MeOH, average specific uptake rate of methanol during consecutive methanol pulses; max. q_MeOH, maximum specific uptake rate of methanol during consecutive methanol pulses; Y_X/MeOH biomass yield on methanol; Y_CO2/MeOH, CO₂ yield on methanol; C-balance, sum of biomass and CO₂ yields; q_p, specific productivity of HRP; vol. productivity, volumetric productivity of HRP; efficiency factor; efficiency of the conversion of substrate methanol into product HRP.

Quick links

Search