Table 5 Oligonucleotide primer list. Primers used for the amplification of upstream and downstream sequences of the OCH1 locus from genomic DNA, for amplification of the whole OCH1 locus for Sanger sequencing, for amplification of fragments to assemble pPpT4_BamHI_OCH1rescue and to amplify the OCH1 ORF
primer name | sequence (5′ - 3′) |
|---|---|
OCH1-5int-fw1 | GAACTGTGTAACCTTTTAAATGACGGGATCTAAATACGTCATG |
OCH1-5int-rv1 | CTATTCTCTAGAAAGTATAGGAACTTCGGCTGATGATATTTGCTACGAACACTG |
OCH1-3int-fw1b | GTTCCTATACTTTCTAGAGAATAGGAACTTCGCGAGATTAGAGAATGAATACCTTCTTCTAAGCGATCG |
OCH1-3int-rv1b | GAAGTATTAGGAGCTGAAGAAGCAGAGGCAGAG |
OCH1check-fw1 | CACACATATAAAGGCAATCTACG |
OCH1check-rv2 | CAATAACTTCTGCAATAGACTGC |
OCH1rescue-fw1 | TTCATAGGCTTGGGGTAATAG |
OCH1rescue-rv1 | CTTGAGCGGCCGCTTAGTCCTTCCAACTTCCTTC |
OCH1rescue_T4fw | GCATACATTTGAAGGAAGTTGGAAGGACTAAGCGGCCGCTCAAGAGGAT |
OCH1rescue_T4rv | CTATTTCTCTGTCATCTATCTATTACCCCAAGCCTATGAAGGATCTGGGTACCGCAGG |
OCH1-ORF-fw | ATGGCGAAGGCAGATGGC |
OCH1-ORF-rv | TTAGTCCTTCCAACTTCCTTCAAATG |
