Figure 3
Applications of HDEN electroporation devices in delivery of biomolecules.
(A) The performance of DNA plasmids electroporation for various types of cells. The duration and number of pulses are fixed as 100 μs and three. (B) Electroporation of siRNA. Firefly luciferase expression level is depressed to about 20% which is as low as transfection by Lipofectamine 2000. Cells without any treatment serve as negative control (NC), Mock: electric stimulation without siRNA, siLuc: siRNA targeting firefly luciferase. E-80V: electroporation at 80 V, E-100V: electroporation at 100 V, E-120V: electroporation at 120 V. The data are averaged from at least three experiments and the error bars stand for the standard error. Statistically significant differences in luciferase activity between Mock and electroporation groups are observed (***P < 0.001). (C) The tumor cell killing efficiency of bleomycin electrochemotherapy. The concentration of bleomycin is varied from 10−6 M to 10−4 M. The data are averaged from at least three experiments and the error bars stand for the standard error. Differences in cell viability are statistically compared between 0 M and other concentration of bleomycin. **P < 0.01 and ***P < 0.001 are plotted when observed.
