Figure 2
From: The relevance and applicability of oocyst prevalence as a read-out for mosquito feeding assays
Classification of oocyst condition.
Mosquito midguts were dissected in phosphate buffered saline (PBS) and stained using 3SP2-Alexa488 anti-CSP antibodies. After staining, midguts were washed twice with PBS for 10 minutes before being sealed under a glass cover slip with Vaseline petroleum jelly. All oocysts were identified and assigned a condition by two independent microscopists. Intact oocysts were visibly unbroken. Ruptured oocysts and oocyst remnants were visibly broken or degraded. All samples were examined within 8 hours of dissection, but sample condition remained stable for a number of days (stored at 4°C) prior to dissection for re-examination. Three ruptured and three intact oocysts are shown in the figure for demonstrative purposes.
