Figure 2
FSC-DE with ALP assay.
(a) Effect of prospective drug cocktails identified with FSC-DE with ALP assay from 10 million potential candidates for the osteoblastogenesis of D1 cells. The prospective cocktails did not induce in vitro mineralization, despite high ALP activity. (b) Correlation between doses of extrinsic factors in each drug cocktail elicited and used in FSC-DE with ALP assay against ALP expression in D1 cells. Dots: each drug cocktail. All data represent the actual measurement values in the FSC-DE process. The highest concentration codes 9 and 10 of RA were involved in 70% of all FSC-generated cocktails. D1 cells were seeded at 3125/cm2 and the ALP activity was measured on day 3. Note that the prospective drug cocktails in Figure 2a were narrowed using setting gates with the following criteria: BMP-2 code less than 9 and ALP activity above 1.5. (a, b) Bar graphs and dots show the mean with/without s.d. of three independent determinations. Each concentration of extrinsic factors represents the one in drug cocktails and does not include the one in FBS.
