Table 2 Concentration of Osteogenic Factors in Physiologic Serum/Plasma and Differentiation Media

From: Guiding the osteogenic fate of mouse and human mesenchymal stem cells through feedback system control

 

Physiological concentration in mouse serum (plasma)

Physiological concentration in human serum (plasma)

Conventional concentration reported in the literature*

TBi medium (without FBS)

m4T5i medium (without FBS)

m8T11i medium (without FBS)

2% FBS

10% FBS

AA or AA2P (μM)

44.6a, (R1)

51.7a, (R2)

50b, 250b, 238.38a

50b

50b

50b

0.35acf, (R3)

1.75acg, (R3)

VD3 (nM)

0.102(R4)

0.1(R5)

10

0

0

0.08

0.0008cf, (R6)

0.0039cg, 6)

BMP-2 (ng/mL)

1(R7)

0.09(R8)

100

100

12.5

0.39

0.012f, (R9)

0.06g, (R9)

Hep (μg/mL)

-

1 ~ 2.4c(R10)

10

0

0

0.39

-

-

RA (nM)

~2(R11), 3.6(R12)

4.6(R12), ~14(R13)

1000

0

0

0

0.72 ~ 1.26f, (R14)

3.6 ~ 6.2(R14)

GC or Dex (nM)

87 ~ 320cd, (R15, 16)

200e, (R17)

10

100

0.39

200

1.7cd, f,(R18)f, (R18), 4cef, (R18)

8.5cdg, R18), 0ceg, (R18)

Serum phosphate or beta-GP (mM)

2.7h, (R19)

0.8 ~ 1.4h, (R20)

10

10

10

10

0.046hf, (R21)

0.23hg, (21)

  1. aAs AA.
  2. bAs AA2P; The effective concentration of AA2P for human osteoblast-like cell is similar to that of AA (R22).
  3. cIn plasma.
  4. dAs corticosterone.
  5. eAs cortisol (hydrocortisone).
  6. fOne-fifth or fiftieth of the original number in the reference.
  7. gOne-tenth of the original number in the reference.
  8. hAs serum phosphate or phosphorus.
  9. iBasal media with each drug cocktail; At MSC osteogenic differentiation assay, these media contained 2 and 10% FBS.
  10. *References in the main text and Supplementary references of osteogenic factors.
  11. (R number): Reference number in the Supplementary reference list for Table 2.
  12. GC: Glucocorticoid. The efficacies of the two hormones for osteoblastogenesis are similar to that of Dex (R23, R24).
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