Figure 2

Structure of the CDT1 gene.

(a) In the schematic diagram of the quail CDT1 gene, the ten exons are depicted as boxes with the coding region shown in black. The structure of a cDNA clone encoding the 588-amino acid Cdt1 protein is shown above. (b) Nucleotide sequence alignment of the quail (q) and chicken (ck) CDT1 5′ regulatory regions (accession nos. KF494239, NC_006098.2). Identical nucleotides are shaded in yellow, the conserved distal (d) and proximal (p) canonical Myc binding sites (E-boxes) are boxed in red, the transcription start (TS) sites in black. The positions of the 5′- and 3′-primers (pr.) used for ChIP and quantitative (qnt.) ChIP are underlined. (c) Schematic diagram of the quail (Coturnix japonica), chicken (Gallus gallus), mouse (Mus muculus) (accession no. NT_078575) and human (Homo sapiens) (accession no. AC092384) CDT1 promoters. Transcription start sites (arrows) and the positions of possible binding sites for Myc (canonical, non-canonical) or E2F are indicated.