Figure 1
Effects of HS-173 on the proliferation of hepatic stellate cells (HSCs).
(A) Cytotoxic effects of HS-173 and LY294002 on HSC-T6 and LX-2 cells were measured using an MTT assay. HSCs were seeded in 48- or 96-well culture plates. After incubating for 1 day, the cells were treated with various concentrations of HS-173 (1–10 μM) or 0.1% dimethyl sulfoxide (DMSO) as a negative control. After incubating for 24, 48 and 72 h, the cells were subjected to the MTT assay. Data are presented as the mean ± S.D. from triplicate wells. Effect of HS-173 on HSC proliferation was measured by (B) BrdU and (C) α-SMA staining, which were photographed at 400 × magnification. The representative image of BrdU-positive cells and α-SMA positive cells were shown with 1 μM HS-173 treatments in HSC-T6 and LX-2 cells. Five independent areas in each slide were imaged and counted in triplicate and expressed as a mean percentage BrdU-positive cells or α-SMA-positive cells/total cells (DAPI). *P < 0.05 and **P < 0.01 versus the control.
