Figure 3 | Scientific Reports

Figure 3

From: HS-173, a Novel PI3K Inhibitor, Attenuates the Activation of Hepatic Stellate Cells in Liver Fibrosis

Figure 3

Effect of HS-173 on HSC apoptosis.

(A) The induction of apoptosis by HS-173 (0–5 μM) was monitored by TUNEL staining (200 × magnification). The representative image of TUNEL positive cells were shown with 5 μM HS-173 treatments in both HSC-T6 and LX-2 cells (B) The expression of cleaved caspase-3, Bcl-2 and β-action was measured by Western blotting in HSC-T6 cells treated with HS-173 at the indicated doses for 24 h. (C) Ratio of red and green fluorescence intensity from JC-1 staining after the treatment with various HS-173. The representative image was shown with 1 μM HS-173 treatment. Data are presented as the mean ± S.D. of three independent experiments. *P < 0.05 and **P < 0.01 versus the control.

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