Figure 4 | Scientific Reports

Figure 4

From: HS-173, a Novel PI3K Inhibitor, Attenuates the Activation of Hepatic Stellate Cells in Liver Fibrosis

Figure 4

Effect of HS-173 on the expression of fibrogenic proteins in HSCs.

(A) After treatment with 1 μM HS-173 for 4 h, the expression of fibronectin and vimentin was detected by immunofluorescence. DAPI was used to visualize the nucleus. Photographs were taken at 400 × magnification. (B) After treatment with various concentrations of HS-173 (0.1–5 μM) for 24 h, effect of HS-173 on the levels of collagen I and TIMP-1 was determined by Western blotting. (C) Immunofluorescent signals corresponding to the expression of collagen IV and MMP-2 in HSCs treated with HS-173 (0.1–5 μM) for 4 h. An image representative of three independent experiments is shown.

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