Figure 5
From: Helium Ion Microscopy (HIM) for the imaging of biological samples at sub-nanometer resolution
HIM imaging of Pristionchus pacificus.
(a) Native imaging of the nematode revealed the interior mouth cavity and tooth structures were obscured by a sheath-like structure. (b) neon milling of the outer sheath in the HIM revealed the primary tooth and DEGO structure of the nematode with minimal thermal damage to the cutting surface. (c) The removal of the outer sheath was performed in a very controlled manner with a final dose of 30 nC/μm3. (d) The delicate nature of the neon milling was demonstrated by the patterning of a small horizontal line using a dose of 0.3 nC/μm3. Despite being strong enough to penetrate the outer cuticle of the nematode, minimal ablation and thermal damage is visible on the surrounding area. Scale Bars: (a–c) 5 μm (d) 1 μm.
