Figure 3
From: Aberrant CDK4 Amplification in Refractory Rhabdomyosarcoma as Identified by Genomic Profiling
ALK expression and fusion assay.
(A). ALK immunohistochemistry (2A-1, positive in the present case, 2A-2, negative control) and ALK FISH (2A-3) of the primary tumor: ALK protein overexpression was confirmed, but ALK rearrangement was not detected. (B). ALK RNA expression in tumor cells from pleural metastasis (sample#2): ALK RNA overexpression was detected. The ALK expression has been normalized to that of 4 housekeeping genes. Lung cancer cell lines (NCIH3122, NCIH2228 and A549) were used as controls for ALK RNA expression and EML-ALK fusion detection. (C). ALK fusion assay using NanoString: no EML4-ALK RNA was detected in the sarcoma specimen.
