Table 1 Somatic variants detected in rhabdomyosarcoma (RMS; primary tumor, sample #1) by whole exome sequencing

No	Ch	position	gene name	ref	var	vac	tr	vrf	vac	tr	vrf	func. Impact	fi score	prediction	score
1	19	35524413	SCN1B	A	G	0	35	0.0%	11	21	52.4%	low	1.865	D	0
2	7	113519206	PPP1R3A	A	T	0	102	0.0%	66	142	46.5%	neutral	0	D	0.03
3	4	94411875	GRID2	C	A	0	61	0.0%	31	67	46.3%			D	0
4	15	29346353	APBA2	G	A	0	62	0.0%	23	53	43.4%	medium	2.28	D	0.02
5	2	219513493	ZNF142	C	T	0	24	0.0%	9	21	42.9%	low	0.875	T	0.3
6	1	53347155	ZYG11A	G	T	0	53	0.0%	18	43	41.9%			D	0
7	16	7568302	RBFOX1	C	A	0	74	0.0%	37	93	39.8%	medium	2.595	D	0
8	2	85533345	TCF7L1	G	A	0	23	0.0%	6	19	31.6%	medium	2.25	T	0.05
9	17	80445942	NARF	A	G	0	23	0.0%	8	28	28.6%	neutral	0.455	D	0.03
10	16	85698723	KIAA0182	T	A	0	24	0.0%	4	16	25.0%	low	1.32	D	0.01
11	X	107224952	TEX13B	G	A	0	47	0.0%	12	54	22.2%	low	1.5	T	0.06
12	11	1093375	MUC2	C	T	0	26	0.0%	4	18	22.2%			T	0.23
13	21	45877015	LRRC3	A	C	0	28	0.0%	4	20	20.0%	low	0.865	T	0.11
14	1	24663626	GRHL3	A	C	0	26	0.0%	4	22	18.2%	low	1.67	D	0
15	19	9005714	MUC16	A	C	2	58	3.4%	8	44	18.2%	medium	1.935	T	0.13
16	18	29178556	TTR	G	C	0	28	0.0%	4	23	17.4%	low	1.545	D	0
17	X	47069419	UBA1	G	C	0	41	0.0%	6	36	16.7%	medium	3.34	D	0
18	11	61563225	FEN1	T	G	0	29	0.0%	5	32	15.6%	medium	2.83	D	0
19	17	12896247	ELAC2	A	C	0	26	0.0%	4	26	15.4%	low	1.87	D	0
20	2	204045181	NBEAL1	A	C	0	72	0.0%	9	66	13.6%	high	4.49	D	0
21	11	117342607	DSCAML1	A	C	0	37	0.0%	5	37	13.5%	medium	2.93	D	0
22	5	140188268	PCDHA4	T	G	0	39	0.0%	5	37	13.5%	medium	2.905	D	0
23	1	145601821	POLR3C	G	C	0	39	0.0%	5	37	13.5%	low	1.39	D	0.04

Ch, chromosome; ref, reference; var, variant; vac, variant allele count; tr, total read; vrf, variant read frequency; D, damaging; T, tolerated; SIFT, Sorting Tolerant From Intolerant (Nucleic Acids Res 2003;31:3812).
Nos. 1, 2, 3, 4, 5, 6, 7, 8, 11 and 21: somatic mutations that were validated by Sanger sequencing (Table 2).
No. not mentioned above: somatic mutations that were not validated by Sanger sequencing.

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