Figure 6 | Scientific Reports

Figure 6

From: Pharmacologic IKK/NF-κB inhibition causes antigen presenting cells to undergo TNFα dependent ROS-mediated programmed cell death

Figure 6

IKK suppression results in increased ROS production and secondary loss of ΔΨm.

(A) FSDC were treated with 10 μM Compound A and at the indicated time points, MitoSOX (red) and DiOC6(3) (green) were analyzed by fluorescent microscopy. Cells with apoptotic morphology are marked with yellow arrows. (B) FSDCs were treated with 10 μM Compound A. These FSDCs were either grown in serum alone or pretreated with 200 μM BHA. At 3, 7, 15 and 24 hours, flow cytometry was performed to analyze staining with MitoSOX Red [ROS production] and DiOC6(3) [mitochondrial membrane potential, ΔΨm]. The results are representative of 3 independent experiments.

Back to article page