Figure 7
ROS production is upstream of caspase activation in NF-κB-inhibition-induced APC death.
(A) FSDC were untreated or pretreated for 1 hr with BHA and samples were collected at 0 hours (no CmpA) or 4, 5, 6 and 7 hours post-treatment with CmpA (10 μM) and caspase-8 and caspase-3 activation were evaluated via immunoblot. β-actin was used as a loading control. All samples were run on the same gel. The full-length gels are provided in the supplemental information. (B) MTT assays were used to measure survival of FSDC that were untreated or treated with the listed doses of CmpA, or IKKiVII in the presence of 200 μM BHA. *, p<0.004; **, p<0.00002 (3 independent experiments in triplicate). (C) FSDC, BMDM and BMDC were treated with CmpA ± 200 μM BHA and analyzed for survival via MTT assay. *, p<0.00005; **, p<0.0002; ***, p<0.001 (3 independent experiments in triplicate). (D) Survival of WT macrophages was measured by MTT in cells treated with the Media or CmpA ± LPS 100 ng, in the presence of 500 μM DFO or 200 μM BHA. *, p<0.02; **, p<0.001 (3 independent experiments in triplicate).
