Figure 1
Reduced expression of Grin2a and Grin2b in Tspyl2 mutant hippocampus.
(A), Nissl staining of coronal brain sections from 2 month-old mice. Cellular composition of hippocampal substructures and layer development of cortex were indistinguishable between wild-type and mutant. Scale bars: 100 μm. (B), The protein level of key glutamate receptors in 2-month old hippocampi was detected by western blot and quantitated by densitometry. Cropped gel images are shown and the gels were run under the same experimental conditions. The protein level was normalized to actin and the wild-type level was set as 1. The protein levels of GluN2A and GluN2B were significantly reduced (n = 4 mice per genotype). (C), Transcript levels of the above genes in 2- month old hippocampi were detected by quantitative RT-PCR. Relative mRNA level to Hprt in the wild-type was set as 1. Expressions of Grin2a and Grin2b were reduced significantly in the mutant (n = 4 mice per genotype). Expression of Reln was unchanged. (D), RNA stability of Grin2a and Grin2b was determined by adding 10 μg/ml actinomycin D to hippocampal cultures at 7 days in vitro. Quantitative RT-PCR was done on samples collected at 0, 4, 8, 16 and 24 hr after treatment. No difference between wild-type and mutant neurons was observed (n = 3 samples per genotype). Error bars represent SEM. *P < 0.05, Student's t-test. Abbreviations: +/Y, wild-type male; m/Y, mutant male; WT, wild-type.
