Figure 2
From: Promotion of quality standard of herbal medicine by constituent removing and adding

Results of identification of the active constituents.
(a) Heat flow power-time (HFP-t) curve for control S. dysenteriae cultured in L.B. culture medium alone. (b, c) Effects of the R. coptidis extract, removed constituents and negative samples on HFP-t curves of S. dysenteriae growth. (d) Contributions of the removed constituents and their corresponding negative samples to the bacteriostatic activity of R. coptidis. (b–d) Control: S. dysenteriae alone; reference: R. coptidis extract (0.8 mg/mL); removed samples: BER+, COP+, EPI+, PAL+ and (JAT + COL)+; negative samples: R. coptidis extract lacking COP (COP−), EPI (EPI−), PAL (PAL−) and JAT + COL combined (JAT + COL)−. The measurements of relative inhibition ratio were performed in triplicate and error bars represent standard error of the mean. P value compared to R. coptidis extract determined by two-way ANOVA.