Table 2 Thermokinetic characteristics of R. coptidis (200 μg/mL) and samples containing different concentrations of COP on S. dysenteriae growth at 37°C (n = 3, Mean ± SD)

C (μg/mL)	k₁ (min⁻¹)	k₂ (min⁻¹)	t₁ (min)	p₁ (μW)	t₂ (min)	p₂ (μW)	I (%)	P (U/g)	W (%)
Control	0.107 ± 0.015	0.020 ± 0.002	73.7 ± 1.3	371.4 ± 3.6	150.7 ± 1.1	470.0 ± 3.3	0.00	–	–
R. coptidis	0.090 ± 0.009	0.017 ± 0.001	75.7 ± 1.0	376.5 ± 2.2	170.0 ± 2.3	359.5 ± 2.2	17.96	–	–
0^§	0.101 ± 0.010	0.018 ± 0.003	73.7 ± 0.5	387.2 ± 1.1	158.3 ± 0.5	467.6 ± 3.0	11.33	–	0.00
8^§	0.096 ± 0.008	0.016 ± 0.004	73.7 ± 0.9	391.2 ± 2.4	165.7 ± 0.9	413.9 ± 4.1	13.20	23.4	4.05
16^§	0.079 ± 0.005	0.013 ± 0.002	78.7 ± 0.7	411.8 ± 1.5	182.3 ± 1.4	321.0 ± 3.4	34.20	142.9	7.79
32^§	0.065 ± 0.002	0.006 ± 0.001	79.3 ± 1.5	296.7 ± 3.2	214.3 ± 1.9	225.9 ± 2.6	71.00	186.5	14.45
64^§	0.054 ± 0.007	0.005 ± 0.002	87.0 ± 1.9	317.5 ± 3.5	262.0 ± 1.7	186.2 ± 1.7	75.22	99.8	25.26
128^§	0.046 ± 0.003	0.004 ± 0.001	120.0 ± 2.4	152.4 ± 2.8	442.7 ± 3.1	88.6 ± 1.8	80.52	54.1	40.33

C: concentration (μg/mL), k₁ and k₂: the growth rate constants in the first and second exponential phases, t₁ and t₂: the appearance time of the first and second highest peaks, p₁ and p₂: the heat flow power (HFP) of the first and the second highest peaks, I: inhibition ratio in stage k₂, P: relative potency of COP, W: content of COP in the added sample, W = W_COP/(W_COP + W_COP⁻) × 100%, where W_COP is the added weight of COP, W_COP⁻ is the weight of R. coptidis extract (200 μg/mL) with COP removed [W_COP⁻ = (1–the content of COP in the R. coptidis extract) × 200 μg/mL]. ^§Concentration of COP in the negative sample. The above measurements were performed in triplicate and error bars represent standard error of the mean.

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