Figure 3
From: Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis
qRT-PCR of genes related to lipid metabolism and mitochondrial functions and eriocitrin reduced lipid accumulation in HepG2 cells.
To confirm the DNA microarray analyses, qRT-PCR was conducted. Genes related to lipid metabolism in zebrafish (a) and HepG2 cells (b). Genes related to mitochondrial biogenesis and respiratory function in zebrafish (c) and HepG2 cells (d). All values are means ± SEM; n = 5, *P < 0.05, **P < 0.01. (e) Oil Red O staining of HepG2. (f) Absorbance of Oil Red O during lipid accumulation. Eriocitrin reduced lipid accumulation in palmitate-stimulated HepG2. All values are means ± SEM; n = 8, *P < 0.05.
