Figure 4
From: Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis
Eriocitrin increased mitochondrial biogenesis and ATP production.
(a) Eriocitrin (10 μM) increased mitochondrial size (red) of HepG2 cells using MitoTracker Red CMXRos staining. Blue colour represents the nucleus (Hoechst 33342). (b) Quantitative analysis of mitochondrial staining. (c) Quantification of mtDNA was accomplished by calculating the ratio of CYTB to nuclear PK and expressing it as mtDNA copy number per cell. (d), (e) ATP quantification with eriocitrin administration. Upon 72 h administration of eriocitrin, there were increased intracellular ATP in HepG2 cells (d) and systemic ATP of 7 dpf zebrafish (e). All values are mean ± SEM; n = 8, *P < 0.05, **P < 0.01.
