Figure 2
From: Discovery of a Structural-Element Specific G-Quadruplex “Light-Up” Probe
GQR reacts with 93del to give a turn-on fluorescence response.
Fluorescence spectra of GQR (10 μM) upon incubation with serial dilutions of 93del (from 0–80 μM) in buffer (20 mM K2HPO4/KH2PO4, 100 mM KCl, pH 7.0). λex: 360 nm. ΦF (without 93del) = 0.014, ΦF (in 40 μM 93del) = 0.28. Inset: photographic image of GQR (10 μM) in the presence of 93del (from 0–40 μM) and data plot of fluorescent emission intensity upon addition of 93del (from 0–80 μM). F0 and Fmax are the fluorescent maximum intensities of the GQR in the absence and presence of 93del respectively. Kd = 25.18 ± 0.02 μM (one site specific binding model). Values are represented as means (n = 3). Measurements were taken at room temperature (RT).
