Figure 2

Intracellular ROS levels in iPS cells.

(A) Intracellular ROS in the iPS cells was loaded with 10 μM 2′,7′-dichlorodihydrofluorescein diacetate for 60 min and representative images showed relatively lower fluorescence intensity in the iPS cell colonies cultured with antioxidants than that of control. Data of semi-quantitative analysis on the intracellular ROS in 201B7 and 253G1 iPS cells were presented from three separate experiments. (B) The intracellular ROS were also determined by flow cytometry and data were presented from three separate experiments. Abbreviations: AOS, proprietary antioxidant supplement from Sigma-Aldrich; AOH, Homemade antioxidant cocktail.