Figure 2

Prospective isolation and antigenic characterization of cells sorted from the adult SEZ according to EGFR and Prominin-1 expression.

(A) Representative examples of FACS plots illustrating the distribution of all the dissociated SEZ (a, g) and the named populations (b–f) according to forward (FSC), side (SSC) scatter and fluorescence levels as indicated. For primary and secondary clones the quantification of clone size is shown in (h) and of the percentage of plated cells forming clones is given in (i). Secondary clones were analysed only in P⁺/E^h and P⁻/E^h cells. Values are the percentage means ± SEM. n = 3. * indicates significantly different from the P⁺/E^h counterpart, t-test. (B) Quantitative analysis by qPCR of the given transcripts in the mentioned populations sorted according the indicated gate-sorting procedures. Values are the means of relative expression levels from ddCT ± SEM normalized to P⁻/E^l cells. n = 3, ANOVA.