Figure 5
From: Proliferation and cilia dynamics in neural stem cells prospectively isolated from the SEZ
Analysis by SEM of precursors in the adult SEZ.
Representative SEM images of the indicated sorted populations (A) and of the apical surface of the SEZ in whole-mount (B). Arrows and arrowhead point to uniciliated cells with seemingly a long (arrows) and a short (arrowhead) cilium. Asterisk points to two closely localized long cilia, which may belong to a biciliated E2 cell. Higher magnification view of the inset in (a) and a detail of the tips of cilia in ependymal multiciliated E1 cells are shown in (a′) and (c), respectively. Scale bars: 1 um and 200 nm in higher magnification views. (C) Quantitative analysis of the percentage of DiI+ labeled cells (a) and relative clonogenic activity (b) in sorted P+/Eh and P−/Eh populations. n = 3. * indicates significantly different from the P−/Eh counterpart, ANOVA.
