Figure 3
From: Wharton's Jelly human Mesenchymal Stem Cell contact guidance by noisy nanotopographies
Actin cytoskeleton polarization.
(a) Representative fluorescence images of actin cytoskeleton acquired at t = 24 h after cell seeding. A schematic of the substrate geometry is shown for each panel. Scale bar = 20 μm. (b) Percentage of cells with aligned actin (0° < αactin < 15°) vs noise. (c) Single cell actin dispersion vs noise. Five independent experiments were performed. (*P < 0.05, ***P < 0.001, ****P < 0.0001).
