Figure 4

Focal adhesions morphology.

(a) Representative fluorescence images of vinculin clusters on selected substrates (T1, 40% noise and FLAT). Scale bars = 20 μm. (b) Schematic of WJ-hMSC focal adhesion. FAs were modeled as ellipses, for which major (d^FA_max) and minor axes (d^FA_min) and directionality (α^FA) were measured. (c) Area (A^FA) (d) minor axis (d_min^FA) and (e) major axis (d_max^FA) vs noise. Three independent experiments were performed. (* P < 0.05).