Figure 6
From: Wharton's Jelly human Mesenchymal Stem Cell contact guidance by noisy nanotopographies
Analysis of cell migration.
Cells were cultured for 24 h under standard conditions and then incubated with Syto 16 dye for nucleus staining. Migration was sampled every 15 minutes for 15 hours. (a–b) Representative tracks of cells moving on T1 and FLAT surfaces, respectively. (c) Measurement schematic. (d) Percentage of steps aligned (dS∥) to the pattern direction (0°–15°) and (f) relative speed (V∥). (e) Percentage of steps perpendicular (dS⊥) to the pattern directionality (75°–90°) and g) relative speed (V⊥). Four independent experiments were performed. (* P < 0.05).
