Figure 3
1-octen-3-ol triggers increased induction of NOS in the exposed hemocytes but not in glial cells.
(a,b) The GFP labelled repo-positive glial cells (repo-GAL4; UAS-eGFP) failed to show any co-localization with NOS positive cells although exposure of 1-octen-3-ol intensified the GFP signal of repo-positive glial cells (arrowheads). (c,d) The hemocytes from the 3rd instar stage larvae of He-GAL4; UAS-eGFP upon exposure to 1-octen-3-ol for 1 hr demonstrated stimulation of NO by hemocytes which co-localize with H2 specific antibodies where the insets demonstrate the morphology and size of the co-localized cells in control and exposed brain (e,f). (g) The mean fluorescence pixel intensity (MFPI) of NOS was elevated in hemocytes exposed to 1-octen-3-ol. (n = 100–150 hemocytes;** = P < 0.01) and represents a significant difference between control and octen-3-ol exposed larvae.
