Figure 5

1-octen-3-ol induces NOS expression in larval tracheal tissues and remodels tracheal epithelial linings.

(a,b) The increased expression of NOS was seen in the epithelial linings of exposed tracheal primary and secondary branches as compared to that of control larvae. (c) The increase in NOS intensity as assessed with mean fluorescence intensity for NOS was detected in 1-octen-3-ol exposed larval tracheal linings (n = 20–25) (d,e) The btl>GFP stained with anti-H2 (hemocytes) and anti-NOS antibodies display co-localization (shown with arrows) between hemocytes, NOS and tracheal epithelial lining in exposed larvae but tracheal linings of control larvae fail to show any measurable co-localization. (n = 10–14) (f,g) The increased numbers of DAPI-positive nuclei (shown with arrows) were detected in the distal branches of tracheal linings of exposed larvae but not in control tracheal linings (n = 8–10).