Figure 3
From: Hsa-miR-520d induces hepatoma cells to form normal liver tissues via a stemness-mediated process
Metabolomic analysis was performed in 520d-HLF cells (5D) that formed malignant hepatoma cells in vivo, 520d-HLF cells (7D) that did not form hepatoma cells in vivo and R1 and R2 cells sorted from 520d-HLF according to GFP and ALP expression (n = 4).
A heat map analysis of three cell types (7D, R1 and R2 against 5D) is shown. Red or yellow columns indicate quantitative changes of greater than 2.0-fold or 1.5–2.0-fold, respectively. In the analyses performed according to this method, no metabolites were reduced quantitatively in 7D, R1 or R2 cells, compared with 5D cells. The metabolomic patterns were similar to those in iPSCs. The profiles of the 7D and R1 cells showed high similarity to each other and glycolysis, the SAM cycle and nucleotide synthesis were more elevated in 7D, R1 and R2 than in 5D cells. Microarray analysis between R1 (cell populations forming liver tissue) and iPS was performed and shown in Supplementary Fig. 9–11 (n = 1) for reference only (TORAY, Kamakura, Japan).
