Figure 1
From: In vivo imaging of kidney glomeruli transplanted into the anterior chamber of the mouse eye
Imaging of isolated mouse glomeruli transplanted into the anterior chamber of the mouse eye.
(a) Schematic drawing of the transplantation procedure of isolated glomeruli into the anterior chamber. (b) For microscopic imaging, recipient mice were anesthetized and restrained with a stereotactic head holder with the transplanted eye facing the objective. (c) Transplanted glomeruli attached to the iris are detectable by low magnification microscopy. (d) Imaging of an engrafted glomerulus after tail vein injection of tetramethylrhodamine-labeled 2.000 kDa dextran (red) into the recipient mouse revealing perfusion of the transplanted glomerulus. Endothelial cell-specific expression of GFP (green) in the recipient (but not the donor) mouse reveals contribution of recipient endothelial cells to the capillary walls of the transplanted glomerulus. Shown is a maximum projection of a Z-stack of confocal images. Scale bar, 10 μm.
