Figure 2
From: In vivo imaging of kidney glomeruli transplanted into the anterior chamber of the mouse eye
Podocyte-specific expression of cyan fluorescent protein helps visualizing transplanted podocytes and reveals continued nephrin expression.
Transplanted glomeruli from donor mice that express cyan fluorescent protein under the NPHS1 (nephrin)-promoter exhibit blue fluorescence (upper two rows), which was completely absent in negative control glomeruli from wild type mice (lower row). NPHS1-CFP expression helps visualize transplanted podocytes and demonstrates continued nephrin expression by podocytes of transplanted glomeruli. Scale bar, 50 μm.
