Figure 7
From: In vivo imaging of kidney glomeruli transplanted into the anterior chamber of the mouse eye
Simultaneous visualization of donor-derived podocytes and recipient-derived endothelial cells.
(a) Maximum projection of a z-stack acquired by confocal laser scanning microscopy of a glomerulus from a donor mouse with podocyte-specific expression of cyan fluorescent protein into a recipient mouse with endothelial cell specific expression of green fluorescent protein. The image was acquired at day 186 after transplantation. Fluorescently labeled 70 kDa dextran remains confined to the intravascular space. (b) Detail from image (a) showing the red channel only. (c) Subsequent injection of 10 kDa dextran in the same mouse reveals filtration of the low molecular weight dextran with some accumulation next to the intravascular space. (d) Simultaneous visualization of podocytes (blue) and recipient-derived endothelial cells (green) confirms localization of the inter- (arrow in c and d) and subpodocyte space (arrowhead in c and d) between endothelial cells and podocytes. Note that separation of the CFP and the GFP signal is not perfect due to the overlapping emission spectra. Scale bars = 5 μm.
