Figure 8
From: In vivo imaging of kidney glomeruli transplanted into the anterior chamber of the mouse eye
Non-invasive in vivo visualization of glomerular proteinuria.
The same glomeruli have been imaged upon tail vein injection of 70 kDa dextran before (panel (a), upper row) and nine days after (panel (a), lower row) doxycycline-induction of podocyte specific NFATc1nuc transgene expression in transplanted glomeruli, which is known to cause podocyte foot process effacement and proteinuria. Fluorescence intensity was quantified along the capillary wall at several points and normalized to intracapillary flurescence intensity. Plotting of fluorescence intensity against distance reveals increased intensity directly adjacent to the outside of the capillary when comparing baseline measurements (blue) to measurements after proteinuria induction (red) in glomerular capillaries ((b); mean intensity of 8 capillary loops in 4 glomeruli of 2 mice plotted against the distance along the capillary cross section) but not in the doxycycline-resistant iris vasculature of the recipient ((c); intensity plot across an iris vessel). (d) The mean fluorescence intensity directly adjacent to the glomerular capillary wall of 8 capillary loops in 4 glomeruli of 2 mice significantly increased upon induction of podocyte damage with doxyxyxline-induced NFATc1nuc transgene expression. Fluorescence intensity has been measured at a defined distance from the capillary wall (1.5 μm from where the fluorescence intensity had decreased to 50% maximum) and normalized to the maximum fluorescence intensity inside the glomerular capillary.
