Figure 4
From: Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer
Hes1 induces stem-like properties in colon cancer cells.
(A) Hes1 increases the percentages of CD133+ cells in Hes1-overexpressing SW620 and HCT116 cells. Flow cytometric profiles of cells in the presence of isotype control are shown in the bottom panels. (B) Hes1 increases the size of the side population (SP) cells. Flow cytometric profiles of SP cells among the SW620 and HCT116 cell lines after stable expression of Hes1. SP cell profiles in the presence of verapamil are shown in the bottom panels. The percentages of SP cells are indicated. (C) Hes1 induces stem cell-like self-renewal properties. Sphere sizes are shown in the left panels and the numbers of spheres is shown in the right panels. (D) Westen blot reveals an increased expression of Hes1 and CD133 in CD133-positive cells in comparison with CD133-negative cells and an increased expression of Hes1 and CD133 in tumour spheres in comparison with adherent cells in SW620 and HCT116 colon cancer cell lines.
