Figure 6
From: Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer
Hes1 enhances the initial tumour cell population in SW620 cells.
(A) Tumours formed after injection of nude mice with Hes1 (LV-Hes1) or vector control (LV-con) expressing SW620 cells. (B) Tumour growth curves after injection of nude mice with Hes1 (LV-Hes1) or vector control (LV-con) expressing SW620 cells. Once they become palpable, the Hes1 tumour cells grow at a higher rate than the vector control cells in all cases. Differences were not evident when the injected cell number was above 106. However, at 105, 104 and 103 cells, the differences were significant. (C) Tumour weights after injection of nude mice with Hes1 (LV-Hes1) or vector control (LV-con) expressing SW620 cells. Once they become palpable, the Hes1 tumour cells grow at a higher rate than the vector control cells in all cases. This difference becomes more pronounced when the injected cell number is 105, 104 and 103. (D) Expression of Hes1, ALDH1, Bmi-1 in Hes1 (LV-Hes1) or vector control (LV-con) expressing tumours detected by using immunohistochemistry. (F) Tumours become palpable at an earlier timepoint and grow at a faster rate in Hes1-expressing cells compared with vector control cells. Tumour formation was monitored for 24–27 days after injection of nude mice. In the case of injections with 104 cells, all tumours arose in 10 mice for Hes1-expressing cells, whereas only 8 of 10 mice infected with vector control cells formed tumours. When 103 cells were injected, all mice formed tumours in the Hes1-expressing cell group, but only 6 of 12 mice did so in the vector control group.
