Figure 1 | Scientific Reports

Figure 1

From: A novel cell-cycle-indicator, mVenus-p27K−, identifies quiescent cells and visualizes G0–G1 transition

Figure 1

mVenus-p27K− probe identifies quiescent cells.

(A) Various constructs with concatenated mVenus and p27. (B) Typical images of NIH3T3 transfectants (Scale, 200 μm). (C) Representative histograms for DNA contents of NIH3T3 (right) and of the fluorescent positive cells (red or green solid line) and the percentages of the fluorescent positive cells in G1/0 cells (left). (D) Representative histograms (upper) and relative median intensity (%) (Relative F.I.) (lower) of fluorescence of NIH3T3 in cycling (black) or serum starvation (red). (E–F) Representative Ki-67 staining of NIH3T3 (E) or Ba/F3 cells (F) or Lewis lung carcinoma cells (F) expressing mVenus-p27K-. NC, isotype control.

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