Figure 4

Dll4 exosomes stimulate Notch target genes.

HMVECs were plated on gelatin coated 6 well plate at a density of 2.5 × 10⁵ per well. HMVECs were grown to 80–90% confluency before being starved for 2 hour. They were conditioned with three concentrations of control exosomes and Dll4 exosomes for 8 hours prior to cell extraction and RNA isolation. qPCR was performed to examine the Notch target genes, Hey1 and Hes1. Histograms indicating the relative expression of the genes at three different exosomes concentrations (A) 1 μgmL⁻¹, (B) 5 μgmL⁻¹ and (C) 20 μgmL⁻¹, *** P < 0.01 significant.