Figure 5 | Scientific Reports

Figure 5

From: The complex and specific pMHC interactions with diverse HIV-1 TCR clonotypes reveal a structural basis for alterations in CTL function

Figure 5

Impact of alanine substitutions on FEP and antiviral efficacy.

(A). The FEP simulation results for the predicted TCR-KK10 binding free energy change due to L6A and R2A single mutations and R2AL6A double mutation in viral peptide KK10. (B). The ability of KK10-specific clonotypes to recognize KK10 WT peptide and alanine mutants was tested in the standard 4-h chromium release assay with peptide-loaded HLA-B*2705-expressing GXR cells at an effector/target cell ratio of 1:1.

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