Figure 1
Positional cloning of the API2 gene and molecular nature and position of the api2 and rpl36aa mutations.
(a) A mapping population of 138 F2 plants derived from an api2/api2 × Col-0 outcross was used to define a candidate interval of 850 kb on chromosome 4. The names and map positions of the molecular markers used are indicated. The number of recombinant chromosomes found and the total of chromosomes analyzed are shown in parentheses. Black boxes represent BAC clones. (b) Structure of the API2 and RPL36aA paralogous genes, with indication of the nature and position of the api2 and rpl36aa mutations. A triangle indicates the position of a T-DNA insertion. Boxes and lines between boxes indicate exons and introns, respectively. Open boxes correspond to the 5′ and 3′ untranslated regions. The predicted translation start (ATG) and stop (TAA) codons are indicated.
