Figure 3

Expression of the API2 and RPL36aA genes in the Col-0 wild type and the api2/api2 and rpl36aa/rpl36aa mutants.
Real-time qRT-PCR analysis of the expression of the API2 and RPL36aA genes was performed in api2/api2 and rpl36aa/rpl36aa vegetative leaves. Relative expression levels are shown, determined by the comparative CT method and normalized with the expression of the 18S rRNA housekeeping gene. Error bars indicate the interval delimited by 2−(ΔΔCT± SD). Asterisks indicate ΔCT values significantly different from those of Col-0 in a Mann-Whitney U test (p < 0.01; n = 9).