Figure 4 | Scientific Reports

Figure 4

From: RETRACTED ARTICLE:Type IV collagen α1-chain noncollagenous domain blocks MMP-2 activation both in-vitro and in-vivo

Figure 4

Regulation of TIMP-2 and MT1-MMP expression in α1(IV)NC1 treated endothelial cells.

(a) TIMP-2 levels in α1(IV)NC1 treated conditioned medium from cultured HUVECs (upper blot) and albumin was shown as loading control (lower blot). (b) Cytosolic and membrane bound MT1-MMP levels in α1(IV)NC1 treated HUVECs (upper blot) and actin was shown as a loading control (lower blot). In Panel (a) and (b) western blots were scanned in Cannon scanner, TIMP-2 and MT1-MMP band intensities were quantitated by using NIH image software and the graphs shows relative amounts of proteins.

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