Figure 5
Regulation of VEGF induced angiogenesis in matrigel matrix.
(a) Different conditions of wild type mice matrigel are shown. Arrows point to the blood vessels. E, M and SM represent endothelial cells, matrigel and smooth muscle. Scale bar corresponds to 50-μm. Graphs show number of blood vessels and Hb content quantification from wild type matrigel implants. Values in mean ± SEM are shown where, *p < 0.01 compared to VEGF with and without α1(IV)NC1 (l/h). (b) In-vivo matrigel angiogenesis in α1-integrin null mice from left to right similar to panel a. Lower graphs showing number of blood vessels and Hb content quantification from α1-integrin null mice matrigel implants. *p < 0.01 compared to VEGF with and without α1(IV)NC1 (l/h). The number of blood vessels in the matrigel plugs was counted in 10 fields. (‘l’ and ‘h’ represents low and high doses of α1(IV)NC1).
