Figure 1
From: Functional transformations of bile acid transporters induced by high-affinity macromolecules
Distribution of ASBT due to the treatment of high-affinity binding LHe-tetraD in cells.
(a) Binding affinity of tetrameric deoxycholic acid (tetraDOCA) conjugated LMWH derivative (LHe-tetraD) with ASBT. The dose-response curve of LHe-tetraD binding to immobilized human ASBT protein. (b) Changes in the ASBT membrane expression after LHe-tetraD and sodium taurocholate (TCA) treatment in the MDCK-ASBT cells. To facilitate representation, the blots are cropped and the full-length blots are presented in the supplementary Figure S7. The translocation of ASBT (green) from membrane to cytoplasm was observed in both (c) Caco-2 cells; scale bar, 10 μm and (d) MDCK-ASBT cells; scale bar, 20 μm.
